Evidence for Radical Formation at Tyr-353 in Mycobacterium tuberculosis Catalase-Peroxidase (KatG)
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چکیده
منابع مشابه
The Met-Tyr-Trp cross-link in Mycobacterium tuberculosis catalase-peroxidase (KatG): autocatalytic formation and effect on enzyme catalysis and spectroscopic properties.
Catalase-peroxidases (KatG) are bifunctional enzymes possessing both catalase and peroxidase activities. Three crystal structures of different KatGs revealed the presence of a novel Met-Tyr-Trp cross-link that has been suggested to impart catalatic activity to the KatGs. High-performance liquid chromatographic separation of the peptide fragments resulting from tryptic digestion of recombinant M...
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Mycobacterium tuberculosis KatG catalyzes the activation of the antitubercular agent isoniazid to yield an inhibitor targeting enoyl reductase (InhA). However, no firm biochemical link between many KatG variants and isoniazid resistance has been established. In the present study, six distinct KatG variants identified in clinical Mycobacterium tuberculosis isolates resistant to isoniazid were ge...
متن کاملAccess channel residues Ser315 and Asp137 in Mycobacterium tuberculosis catalase-peroxidase (KatG) control peroxidatic activation of the pro-drug isoniazid.
Peroxidatic activation of the anti-tuberculosis pro-drug isoniazid by Mycobacterium tuberculosis catalase-peroxidase (KatG) is regulated by gating residues of a heme access channel. The steric restriction at the bottleneck of this channel is alleviated by replacement of residue Asp137 with Ser, according to crystallographic and kinetic studies.
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Background The mycobacterial catalase-peroxidases protect it from the reactive oxidative metabolites and allow its survival within the host phagocytes. We compared the virulence of Mycobacterium avium complex (MAC) clinical isolates with varying catalase activity recovered from the blood of HIV patients in terms of invasiveness and intracellular multiplicity in host cells. The catalase activity...
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†Departamento de Quı́mica, Universidade Federal de Lavras—UFLA, Campus Universitário, Lavras, Caixa Postal 3037, Lavras, MG CEP: 37200-000, Brazil ‡Centro Brasileiro de Pesquisas Fı́sicas (CBPF), Rua Dr. Xavier Sigaud, 150, Urca, Rio de janeiro, RJ 22290-180, Brazil {Departamento de Quı́mica Orgânica, Instituto de Quı́mica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, CT, Bl. A, Lab. 609...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m311884200